February 2018 edition of the Whole Dog Journal
…has a great article on Fecal Transplants…. well worth reading!
2014… Fecal Transplantation….a new old possibility …..
5 December 2014, Pages 463–473
Prevalence of Clostridium perfringens, Clostridium perfringensenterotoxin and dysbiosis in fecal samples of dogs with diarrhea
Clostridium perfringens is commonly recognized as a cause of diarrhea with mucus and blood in the dog. The purpose of this study was to determine if fecal transplantation could be used to cure Clostridium perfringnes infections that were not cured by treatment with Metronidazole and Amoxicillan trihydrate/clavulanate potassium.
C. perfringens was detected in all dogs in this study.
Detection was not indicative of the presence of C. perfringens enterotoxin (CPE).
Fecal dysbiosis was significantly associated with GI disease.
Dysbiosis was independent of the presence of enterotoxigenic C. perfringens or CPE.
Increases in enterotoxigenic C. perfringens may be part of intestinal dysbiosis.
Clostridium perfringens has been suspected as an enteropathogen in dogs. However, its exact role in gastrointestinal (GI) disorders in dogs remains unknown. Recent studies suggest the importance of an altered intestinal microbiota in the activation of virulence factors of enteropathogens. The aim of this study was to evaluate the relationship between diarrhea, dysbiosis, and the presence of C. perfringens and its enterotoxin (CPE). Fecal samples were collected prospectively from 95 healthy control dogs and 104 dogs with GI disease and assessed for bacterial abundances and the presence of CPE using quantitative PCR and ELISA, respectively. C. perfringens was detected in all dogs. Potentially enterotoxigenic C. perfringenswere detected in 33.7% (32/95) of healthy control dogs and 48.1% (50/104) diseased dogs, respectively. CPE was detected by ELISA in 1.0% (1/95) of control dogs and 16.3% (17/104) of diseased dogs. Abundances of Fusobacteria, Ruminococcaceae, Blautia, and Faecalibacterium were significantly decreased in diseased dogs, while abundances of Bifidobacterium, Lactobacillus, and Escherichia coli were significantly increased compared to control dogs. The microbial dysbiosis was independent of the presence of the enterotoxigenic C. perfringens or CPE. In conclusion, the presence of CPE as well as fecal dysbiosis was associated with GI disease. However, the presence of C. perfringens was not indicative of GI disease in all cases of diarrhea, and the observed increased abundance of enterotoxigenic C. perfringens may be part of intestinal dysbiosis occurring in GI disease. The significance of an intestinal dysbiosis in dogs with GI disease deserves further attention.
- RNA, 16S rRNA 16S ribosomal;
- AHD, acute hemorrhagic diarrhea;
- CPE, Clostridium perfringensenterotoxin;
- cpe gene, Clostridium perfringens enterotoxin gene;
- GI, gastrointestinal;
- qPCR, quantitative real-time polymerase chain reaction;
- RPLA, reverse passive latex agglutination assay;
- SCFA, short-chain fatty acid
- Clostridium perfringens;
Published by Elsevier B.V.
To test for this, please contact Texas A&M Gastrointestinal Lab: http://vetmed.tamu.edu/gilab/service/assays/fecal-elisa-for-c-perfringens-enterotoxin
The following is a veterinary report and actual notes from one of our members, Johnny, who had the Fecal Transplant done on Nov 6, 2014 on his EPI dog, Gretta… with great success.
Gretta had the transplant and all went well. At first there were good days and some not so good. The not so good was few and far between though and eventually her stools looked great. This went on for a few months but the majority of the time she was very good and had very firm stools. Gretta has been very good in the last 3 months or so. I have figured that stress causes he a little loose stool. It seems as much as she likes to ride in the truck and go places it does stress her some. It is not visibly noticeable, but most of her trips when she was a baby were to the vet and she was always car sick so I can see why she is a bit nervous at times.
Today Gretta is fine. I truly believe that the Fecal transplant helped tremendously. It gave her gut good flora where what I suspect was only bad. Plus all the previous antibiotics had taken their toll also. Today Gretta does have a few instances with soft stools, Nothing like what she has had in the past, But as I suspect it is usually after a long ride in the Suburban to go to the beach or just to ride for the day and sight see. The soft stools immediately clear up the next day. Previous to the transplant Gretta’s liver values were a bit high but nothing to worry about. I suspect the combinations of meds had something to do with that. Last month she had her bloodwork done and everything was excellent. Today we enjoy the fact of knowing Gretta is health and happy. No tummy trouble.
……. and one very Happy Gretta!
The articles on the procedure are from VIN.com The web address is http://www.vin.com/members/cms/document/default.aspx?objecttyp… That one is called Use of fecal transplant in eight dogs with refractory clostridium perfringes-associated diarrhea and refers to dogs.
To read current ………
FECAL MICROBIOME PUBLICATIONS
check on this link: http://www.pubfacts.com/search/fecal+microbiome
Well worth checking out… Dr. Margo Roman’s website:
Veterinarian Margo Roman has done over 420 fecal transplants at Main St. Animal Services of Hopkinton in Massachusetts http://mashvet.com/about . She’s created a website about the therapy, with a very funny, slightly naughty, name.
Dr. Roman has lectured about the procedure in Thailand and Japan, and has two papers coming up on micro-biome restorative therapy, which she hopes will become the natural way to reboot the gut. If this procedure can keep the GI tract, a vital organ that contains up to 85 percent of the immune system, working perfectly, then it has the potential to help sick animals become well and apparently healthy animals to achieve optimal health.
What is Micro-biome Restorative Therapy (MBRT)?
MBRT is a treatment used for gastrointestinal ailments, immune endrocrine imbalances, and other varying diseases. This therapy, performed for pets and people, has helped many lives with their chronic conditions. For pets, their chronic ailment may just be solved by eating poop.